UofM Biophysics Faculty and Staff

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Bruce A Palfey

Assistant Professor


Ph.D., University of Michigan

Dept:  Department of Biological Chemistry
Office Address:  4433B Med. Sci. I
Phone:  (734) 615-2452

Email:  brupalf@umich.edu



Biological Chemistry page


Representative Publications


Shi, J., Dertouzos, J., Gafni, A., Steel, D., and Palfey, B. A. (2006) "Single-Molecule Kinetics Reveals New Signatures of Half-Sites Reactivity in Dihydroorotate Dehydrogenase A in Catalysis", Proc. Natl. Acad. Sci. USA, 103, 5775-5780

Fisher, H. F., Palfey, B. A., Maniscalco, S. J., and Indyk,; L. (2006) "The Relationship Between Time-Dependence of a Transient-State Kinetic Isotope Effect and the Location of Complexes in a Reaction Sequence", J. Phys. Chem. A, 110, 4465-4472

Brender, J. R., Dertouzos, J., Ballou, D. P., Massey, V., Palfey, B. A., Entsch, B., Steel, D. G., and Gafni, A. (2005) "Conformational Dynamics of the Isoalloxazine in Substrate-Free p-Hydroxybenzoate Hydroxylase: Single-Molecule Studies", J. Am. Chem. Soc., 127, 18171-18178

Frederick, K. K. and Palfey, B. A. (2005) "Kinetics of Proton-linked Flavin Conformational Changes in p-Hydroxybenzoate Hydroxylase", Biochemistry, 44, 13304-13314

Gattis, S. G. and Palfey, B. A. (2005) "Direct Observation of the Participation of Flavin in Product Formation by thyX-Encoded Thymidylate Synthase", J. Am. Chem. Soc., 127, 832-833

Zheng, Y., Carey, P. R., and Carey, B. A. (2004) "The Raman Spectrum of Fully Reduced Flavin", J. Raman Spectroscopy, 35, 521-524

Shi, J., Palfey, B. A., Dertouzos, J., Jensen, K. F., Gafni, A., and Steel, D. (2004) "Multiple States of the Tyr318Leu Mutant of Dihydroorotate Dehydrogenase Revealed by Single Molecule Kinetics", J. Am. Chem. Soc., 126, 6914-6922

Palfey, B. A., Murthy, Y. V. S. N., and Massey, V. (2003) "Altered Balance of Half-Reactions in p-Hydroxybenzoate Hydroxylase Caused by Substituting the 2'-Carbon of FAD with Fluorine", J. Biol. Chem., 278, 22210-22216

Argyrou, A., Blanchard, J. S., and Palfey, B. A. (2002) "The Lipoamide Dehydrogenase from Mycobacterium tuberculosis Permits Direct Observation of Flavin Intermediates in Catalysis", Biochemistry, 41, 14580-14590

Ottosen, M. B., Björnberg, O., Nørager, S., Larsen, S., Palfey, B. A., and Jensen, K. F. (2002) "The Dimeric Dihydroorotate Dehydrogenase A from Lactococcus Lactis Dissociates Reversibly into Inactive Monomers", Protein Science, 11, 2575-2583

Wu, X., Palfey, B. A., Mossine, V. V., and Monier, V. M. (2001) "Kinetic Studies, Mechanism and Substrate Specificity of Amadoriase I from Aspergillus sp.", Biochemistry, 40, 12886-12895

Zheng, Y., Massey, V., Schaller, A., Palfey, B. A., and Carey, P. R. (2001) "Comparison of Resonance Raman Spectra of Flavin-3,4 Dihydroxybenzoate Charge-Transfer Complexes in Three Flavoenzymes", J. of Raman Spectroscopy, 32, 579-586

Palfey, B. A., Björnberg, O., and Jensen, K. F. (2001) "Specific Inhibition of a Family 1A Dihydroorotate Dehydrogenase by Benzoate Pyrimidine Analogues", J. Med. Chem., 44, 2861-2864

Björnberg, O., Jordan, D. B., Palfey, B. A., and Jensen, K. F. (2001) "Dihydrooxonate is a Substrate of Dihydroorotate Dehydrogenase (DHOD) Providing Evidence for Involvement of Cysteine and Serine in Base Catalysis", Arch. Biochem. Biophys., 391, 286-294

Palfey, B. A., Björnberg, O., and Jensen, K. F. (2001) "Insight into the Chemistry of Flavin Reduction and Oxidation in Escherichia coli Dihydroorotate Dehydrogenase Obtained by Rapid Reaction Studies", Biochemistry, 40, 4381-4390

Frederick, K. K., Ballou, D. P., and Palfey, B. A. (2001) "Protein Dynamics Control Proton Transfers to the Substrate on the His72Asn Mutant of p- Hydroxybenzoate Hydroxylase", Biochemistry, 40, 3891-3899

Altose, M. D., Zheng, Y., Palfey, B. A., and Carey, P. R. (2001) "Comparing Protein-Ligand Interactions in Solution and in Single Crystals by Raman Spectroscopy", Proc. Nat. Acad. Sci. U.S.A, 98, 3006-3011

Palfey, B. A., Moran, G. R., Entsch, B., Ballou, D. P., and Massey, V. (1999) "Substrate Recognition by "Password" in p- Hydroxybenzoate Hydroxylase", Biochemistry, 38, 1153-1158


Book Chapters

Palfey, B. A. (2003) "Mechanisms", Ch. 2A in Enzymes and Their Inhibition: Drug Development (Smith, H. J, and Simmons, C., eds.), CRC Press, pp. 43-66

Palfey, B. A. (2003) "Time Resolved Spectral Analysis", Ch. 9 in Kinetic Analysis of Macromolecules: A Practical Approach (Johnson, K. A., ed.), Oxford University Press, pp. 203-228

Palfey, B. A., and Massey, V. (1998) "Flavin-Dependent Enzymes", Ch. 29 in Comprehensive Biological Catalysis, volume III/Radical Reactions and Oxidation/Reduction (Sinnott, M., ed.), Academic Press, pp. 83-154

Palfey, B. A., Ballou, D. P., and Massey, V. (1995) "Oxygen Activation by Flavins and Pterins", Ch. 2 in Active Oxygen: Reactive Oxygen Species in Biochemistry (Valentine, J. S., Foote, C. S., Greenburg, A., and Lieberman, J. F., eds.), Chapman-Hall, pp. 37-83


      
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