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The Coward Laboratory

TLC Stains
p-Anisaldehyde

1 mL p-Anisaldehyde

2 mL H2SO4

100 mL AcOH

Bromo Cresol Green

0.04 g Bromo Cresol Green

100 mL EtOH

0.1 N NaOH until Blue

Dinitrophenyl hydrazine

100 mg DNP

90 mL EtOH

10 mL conc. HCl

Phosphomolybdic Acid

0.5 g PMA

100 mL EtOH

Ninhydrin

0.3 g Ninhydrin

100 mL n-Butanol

3 mL AcOH

Vanillin

9 g Vanillin

150 mL EtOH

5 mL H2SO4

40 mL H2O

4-(4-Nitrobenzyl)pyridine

2-5 g NBP

100 mL Acetone

Base Dip: 4:1 Acetone/Et3N

KMnO4

1.5 g KMnO4

10 g K2CO3

2.5 mL 5% NaOH

150 mL H2O

Ceric Ammonium Molybdate

5 g CeSO4

25 g (NH4)6Mo7O24 . 4H2O

450 mL H2O

50 mL H2SO4

Last updated: May 2004
Site maintained by
J. Suda, jsuda@umich.edu