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Selected Publications

1. Shi, J., Palfey, B. A., Dertouzos, J., Jensen, K. F., Gafni, A., Steel, D. (2004) Multiple States of the Tyr318 Leu Mutant of Dihydroorotate Dehydrogenase Revealed by Single-Molecule Kinetics J. Am. Chem. Soc.  126(22); 6914-6922.

2. Wisser, K.C., Schauerte, J.A., Burke, D.T., Galecki, A., Chen, S., Miller, R.A. and Gafni, A. (2004) Mapping Tissue-Specific Genes Correlated with Age-Dependent Changes in Protein Stability and Function.  Archives of Biochemistry and Biophysics 432,58-70.
   

3. Rhodes, E., and Gafni, A. (2003) Micelle formation by a fragment of human islet amyloid polypeptide. Biophys. J. 84(5):3480-7.

4. Fisher, C.J., Gafni, A., Steel, D.G., and Schauerte, J.A. (2002) The triplet-state lifetime of indole in aqueous and viscous environments:  significance to the interpretation of room temperature phosphorescence in proteins. J. Am. Chem Soc. 124(35):10359-66.

5. Dirnbach, E., Steel, D.G., and Gafni, A. (2001) Mg2+ binding to alkaline phosphatase correlates with slow chanes in protein lability. Biochemistry 40(37):11219-26.

6. Fischer, C.J., Schauerte, J.A., Wisser, K.C., Steel, D.G., and Gafni, A. (2001) Differences in the pathways for unfolding and hydrogen exchange among mutants of Escherichia coli alkaline phosphatase. Biochem. Biophys. Acta. 1545(1-2):96-103.

7. Fischer, C.J., Schauerte, J.A., Wisser, K.C., Gafni, A., and Steel, D.BG. (2000) Hydrogen exchange at the ore of Escherichia coli alkaline phosphatase studied by room-temperature phosphorescence.  Biochemistry 39(6):1455-61.

8. Dirnbach, E., Steel, D.G., and Gafni, A. (1999) Proline isomerization is unlikely to be the cause of slow annealing and reactivation during the folding of alkaline phosphatase.  J. Biol. Chem.  274(8):4532-6.

9. Lewis, M.K., Wolanin, P., Gafni, A., Steel, D.G. (1998) Near-Field Scanning Optical Microscopy of Single Molecules by Femtosecond Two-Photon Excitation.  Optics Letters 23;1111-1113.
   

10. Schauerte, J.A., Steel, D.G., and Gafni, A. (1997) Time-resolved room temperature tryptophan phosphorescence in proteins. Methods Enzymol. 278:49-71.

11. Hansen, J.E., Steel, D.G., and Gafni, A. (1996) Detection of a pH-dependent conformational change in azuring by time-resolved phosphorescence. Biophys. J. 71(4):2138-43.

12. Subramaniam, V., Steel, D.G., and Gafni, A. (1996) In vitro renaturation of bovine beta-lactoglobulin A leads to a biologically active but incompletely refolded state. Protein Sci. 5(10):2089-94.

13. Schauerte, J.A., Gafni, A., and Steel, D.G. (1996) Improved Differentiation Between Luminescence Decay Components by Use of Time-Resolved Optical Activity Measurements and Selective Lifetime Modulation. Biophys. J. 70:1996-2000.

14. Schauerte, J.A., and Gafni, A. (1995) Photodegradation of Tryptophan Residues and Attenuation of Molecular Chaperone Activity in Alpha-Crystallin are Correlated. Biochem. Biophys. Res. Comm. 212:900-905.

15. Schauerte, J.A., Schlyer, B.D., Steel. D.G., Gafni, A. (1995) Nanosecond Time-Resolved Circular Polarization of Fluorescence: Study of NADH Bound to Horse Liver Alcohol Dehydrogenase. Proc. Natl. Acad. Sci. USA92:569-573.

16. Schlyer, B.D., Schauerte, J.A., Steel, D.G., and Gafni, A. (1994) Time-resolved room temperature protein phosphorescence: nonexponential decay from single emitting tryptophans. Biophys. J. 67:1192-1202.

17. Hansen, J. and Gafni, A. (1994) Fluorescence Detection of Conformational Changes in GroEL Induced by Thermal Switching and Nucleotide Binding.  J. Biol. Chem 269:6286-6289.

18. Mersol, J.V.,Steel, D.G., Gafni, A. (1993) Detection of Intermediate Protein Conformations by Room Temperature Phosphorescence Spectroscopy During Denaturation of Escherichia Coli Alkaline Phosphatase. Biophysical Chemistry 48:281-291.

19. Mersol, J.V.,Steel, D.G., Gafni, A. (1991) Quenching of Tryptophan Phosphorescence in Escherichia Coli Alkaline Phosphatase by Long-Range Transfer Mechanisms to External Agents in the Rapid Diffusion Limit. Biochemistry 30:668-675.