Gafni/Steel Research Projects   

	Single-molecule studies of amyloid-beta oligomer binding on live cells Determine the size distribution of amyloid-beta oligomers which bind to living neural cells at physiological concentrations; Characterize the kinetics of oligomer binding and growth on live cell membranes; Explore possible binding sites of amyloid-beta on the cell; Characterize the motion of membrane-bound oligomers. Techniques: Fluorescence Lifetime Measurement by reverse-mode Time-Correlated Single Photon Counting; single-molecule Confocal Laser Scanning Microscopy; TIRF microscopy single particle tracking; fluorescence colocalization; fluorescent calcium indicator studies.
	Single-molecule studies of amyloid-beta oligomer binding on the model membrane Study the aggregation mechanism of amyloid-beta(1-40), amyloid-beta(1-42), and their mixture on the phospholipid membrane.
	Abeta-liposome interactions
	Single molecule study of Abeta interaction with black lipid membrane
	Single molecule membrane interactions

Former Research Projects:

	Amylin-liposome Interactions
	Movie of Melittin causing leakage of SH-SY5Y cell followed by calcium indicator
	Live cell imaging

Flavin Enzymes.  Mechanism of an electron/proton transfer protein

Single Molecule Studies of p-hydroxybenzoate hydroxylase (PHBH)

Single Molecule Studies of Dihydroorotate Dehydrogenase (DHOD)

Monte Carlo Simulation of Single Molecule Kinetics

Time Resolved Circularly Polarized Phosphorescence Studies

Near Field Spectroscopy Project

Alkaline Phosphatase Research