Fundamental Laboratory Approaches
for Biochemistry and Biotechnology

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by Alex J. Ninfa and David P. Ballou

Notes on Experiments Homework Answers Book Revisions Contact Us Authors Home Links		Chapter 7, page 187. Question 7-1. The acidic side chains of the protein must be ionized in order for the protein to be attracted to the resin. Question 7-2. The sample must be applied to the ion-exchange column in low salt, since the salt will bind to the available sites on the column and prevent the protein from binding. See figure 4-11 on page 111. Indeed, we will use salt to elute the protein from the column.