SOLiD(tm) 3 Plus - Next-Generation Sequencing & Gene Expression Analysis

SOLiD Day Open House - Nov. 4, 2009

8:30 - 10:00 BSRB Lobby Conference Rooms

The genome in high definition: DNA, RNA & Whole Transcriptome applications using SOLiD(tm) 3+ Next Generation Sequencing: Alex Robertson, SOLiD Technical Consultant, Applied Biosystems

11:00 - 2:00 UMMCC Microarray Core, 7410 CCGC

Open House - Meet SOLiD in person

Join us at the Cancer Center for Food, Door Prizes and the latest on how SOLiD can help your research (Lunch RSVP email requested, info below)

2:00 - 4:00 Seminars - Med. Sci. II North Lecture Hall, Rm. 3695
2:00 - 3:00 The genome in high definition: DNA, RNA & Whole Transcriptome applications using SOLiD Next Generation Sequencing: Alex Robertson, SOLiD Technical Consultant, Applied Biosystems, Toronto, ON

3:00 - 4:00 Using SOLiD Next Generation Sequencing to Profile microRNA in cancer samples: Jeff Palatini, Research Director, Microarray Shared Resource (MASR), Comprehensive Cancer Center, The Ohio State University, Columbus, OH

Individual SOLiD 3 Plus expert project consultations are available throughout the day (30 minutes each). E-mail to schedule your appointment.

Note:

ALL total RNA samples submitted to core need to be evaluated on the Agilent 2100 BioAnalyzer prior to being run on Affy or PCR arrays.

Targeted PCR arrays from both Lonza and SuperArray. These pathway specific commercial arrays are only $50 per sample (processing is $80 each) when purchased in the core (sold as a 4 x 96 genes in a 384 well plate). Click on the Pathway Arrays link to the left for more details.

Affymetrix released its Mapping 6.0 GeneChip which includes 1 million SNPs and ~400k non-polymorphic probes (for copy number variation analysis/LOH). UMCCC Affymetrix core offers full service processing on Afffymetrix DNA Mapping arrays. For more info regarding these arrays, click here.

Small Sample Prep: The Core will process RNA samples from as little as 20ng total RNA using the NuGen Ovation Biotin labeling system.

Available in the Core: ABI 7900HT real time PCR instrument. Run real time PCR and/or SNP assays. Call or email to set up a time or for any questions.

Agilent 2100 BioAnalyzer: Available for RNA, DNA, Protien quality/quantity determinations. NanoDrop UV/Vis Spectrophotometer service included with RNA nano.