Affymetrix & Microarray Core

DNA picture

Welcome to the University of Michigan's DNA Sequencing Core Affymetrix and Microarray Core Group. The facility is now located at the NCRC, building 14, room 148. We also maintain a sample QC and drop off location at 2568 MSRB II, phone 4-1461. The MSRB II site is open daily from 9am to 4pm. The core is available campus wide and to external non-profits.

 

Notes: New Online qPCR scheduling.

Next Generation Sequencing samples: The core will now create Whole transcriptome libraries and Exome captured libraries for end users. These can be for either ABI solid or Illumina HiSeq 2000 sequencers.  Customer made libraries can also be run for those PI's looking for faster turnaround times.

ALL total RNA samples submitted to core need to be evaluated on the Agilent 2100 BioAnalyzer prior to being run on Affy or PCR arrays. This is available both at NCRC, and at 2568 MSRB II.

Targeted PCR arrays from both Qiagen (RT Profiler arrays) and ABI (OpenArray). RT Profiler pathway specific commercial arrays are only $159 per sample when processed in the core (sold as a 4 x 96 genes in a 384 well plate). Click on the Pathway Arrays link to the left for more details.  The OpenArray panels are $150 per sample and contain 600-700 genes each.  Click on the Panels here to see a current listing.

Affymetrix Mapping 6.0 GeneChip which includes 1 million SNPs and ~400k non-polymorphic probes (for copy number variation analysis/LOH). The Affymetrix core offers full service processing on Afffymetrix DNA Mapping arrays. For more info regarding these arrays, click here.

Small Sample Prep: The Core will process RNA samples from as little as 10ng total RNA using the NuGen's Biotin labeling systems.

Available in the Core: ABI 7900HT real time PCR instrument. An ABI Step One Plus system is also available. Run real time PCR and/or SNP assays. Please click on ABI/Real Time PCR link (left bar) to schedule your time and view the current usage schedules.

Agilent 2100 BioAnalyzer: Available for RNA, DNA, Protien quality/quantity determinations. NanoDrop UV/Vis Spectrophotometer service included with RNA nano.