Guidelines for Sample Submission

The following are the pre-requisites asked of you prior to bringing samples to the Microarray Core Facility:

1-

Please download and submit with your samples the correct form:

Affy Gene Expression Sample Submission Form

Affy Genome SNP 6.0 Sample Submission Form.

Targeted PCR Array Submission Form

Next-Generation Sequencing Sample Submission Form

Autopure DNA isolation services Submission Form

One of these forms is required. Please call 7-5623 prior to dropping samples off to ensure someone is in the core to accept your samples.

2-

For each sample submitted, you will need to create an unique identifier begining with your investigators last name and a three digit number. (ie. Dr. Macoska's lab would submit their first sample to the core as Macoska_001). Investigators are responsible for maintaining records on which samples correspond to each unique identifier.

3-

Record the corresponding unique numbers on the tubes you bring to the Core Facility and bring the completed submission form. You need to provide us with a current UM shortcode. Please also make sure you are registered with the DNA sequencing core (both PI and lab contact). While we are not using this system for sample submission, this is need for proper billing. We will not process samples unless you bring in the completed form and the PI, lab contact and shortcode is entered into the Seq. core's database.

4-

All samples MUST be checked on the Agilent 2100 BioAnalyzer prior to being run on Affy or on targeted arrays. The core offers the BioAnalyzer as a stand alone service. You are advised to check your samples via a gel and determine concentrations from absorbance A260/280 readings prior to submitting to the core in order to prevent any unnecessary or extra costs to you. Attach Agilent pdf to sample submission form. Please see the FAQ page for further information.

5-

Bring your Affymetrix samples as follows:

Affy Standard - >50 ng/ul and >250ng total RNA

Nugen - >10ng/ul and >50ng total RNA

PCR Array - >0.1ug/ul and >500ng total RNA

Bring us at least 10ul. If your sample or samples do not pass our minimum requirements you will be contacted via email and consulted on how to proceed. You may be charged for additional handling of your samples ($30) so please try to meet the stated criteria.

6-

Please bring samples on ice or dry ice. Call ahead to make sure someone is in the core to accept the samples.

7-

For the ABI Solid:

Total RNA: 5ug total RNA and >500ng/ul ** We are working to bring this down, email seqcore@umich.edu to see a lower conc. will work.

Genomic DNA for exon capture: >3ug and >100ng/ul

8- If you need to ship samples to us, our address is: 2800 Plymouth Rd, Bldg 14, Room 165, Ann Arbor, MI 48109-2800. The phone number is 734-647-5623. Please provide us with a FedEx (or your favorite carrier) account so that we can ship data and samples back to you.

 

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