Microarray Core Facility

Quantitative PCR

Instrument Info

The ABI PRISM 7900HT Sequence Detection System is a high-throughput real time PCR system that detects and quantifies nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay. Pre-developed assays (both Gene Expression and SNP) can be purchased from Applied BioSystems. Download these pdf spec sheets for more instrument details.

ABI 7900HT Overview

ABI StepOne Plus Overview

A review paper - RNA integrity and the effect on the real-time qRT-PCR performance

QPCR plate - any format:  $16.00  per plate.

Scheduling a Time

The core offers 2 or 3 hour blocks of time on each of the qPCR instruments located at 2568 MSRB II or the NCRC.  The end user is expected to set up qPCR reactions in instrument specific plates (0.1mL 96 well semiskirted plates for the Step One Plus, and either 0.2mL 96 well semiskirted or 384 well PCR plates for the 7900HT instrument). The ready to run plates can be dropped off at or before the start time selected when making an appointment.  Appointments are cancelable anytime before the appointment start time. 

To book a time on an instrument, use the link below. You should also be able to view available slots in the instrument schedules below.  If you run into any errors while using the new system, please email ngallagh@umich.edu or call 7-5623.  Thanks for your support during our transition.

Basic supplies and reagents are available at the Biomedical Research Stores.

Related Services:

Recharge Rate Product
 $1.00  Nanodrop Service
Unit price per sample. RNA or dsDNA concentration determination using Nanodrop Spectrophotometer.
 $52.00  RNA 6000 Nano BioAnalyzer
Unit price per chip. Each RNA 6000 Nano chip can run up to 12 RNA or cDNA samples. Results include both a concentration estimate and a RNA Integrity Number (RIN).
 $220.00  Full Service Processing for GeneChips and Array Strips
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix cartridges and array strips only. Requires 300ng total RNA or more.
 $120.00  Full Service Processing for Array Plates
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix Array plates only. Requires 300ng total RNA or more.
 $310.00  Full Service Processing for GeneChips and Array Strips - Limited sample input
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix cartridges and array strips only. Requires 10ng total RNA or more.
 $190.00  Full Service Processing for Array Plates - Limited Sample input
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix Array plates only. Requires 10ng total RNA or more.
 $16.50  DNA isolation from Whole Blood
Unit price per sample. Genomic DNA can be isolated from up to 5mL of fresh whole blood. See link for additional options.