Microarray Core Facility

MicroRNA and Non-coding RNA analysis

Affymetrix miRNA arrays

Since their discovery in 1993, small non-coding RNAs (microRNA, scaRNA, and snoRNA) have emerged as a major component in the regulatory circuitry that underlies the development and physiology of complex organisms. As a result, it has become increasingly important to complement messenger RNA (mRNA) gene expression studies with miRNA analysis in order to understand the biological context of differentially expressed genes. These key functional gene products are estimated to regulate approximately 30 percent of all protein-coding genes. Keeping pace with the discovery of new and novel miRNA, Affymetrix is pleased to announce the release of the NEW Affymetrix® miRNA 3.1 Array Strip—the latest addition to our growing catalog of miRNA products.

Affymetrix miRNA 3.1 Array Overview

() represents the number of samples per array plate.

Affymetrix miRNA 3.1 Array Strip (4):  $500.00  per strip.
Affymetrix Array Processing (1):  $220.00  per sample.

OpenArray miRNA arrays

Each TaqMan® OpenArray® MicroRNA Panel contains 754 well-characterized miRNA sequences from the Sanger miRBase v14. All 754 assays have been functionally validated with miRNA artificial templates.

Human MicroRNA Panel - List of Human microRNA's targeted.
Rodent MicroRNA Panel - List of Rodent microRNA's targeted.

ABI OpenArray miRNA panel (3):  $585.00  per panel.
Full Service Processing (total RNA to data) (1):  $105.00  per sample.
OpenArray Processing only (1):  $60.00  per array.

*** Please note - OpenArray microRNA panels must be run in groups of 3 samples (1 array runs 3 samples).

qPCR

The core also offers access to qPCR systems. Faculty and staff can sign up for time to run 96 well or 384 well plates with assays of their choosing.

qPCR Plate (1):  $16.00  per plate (any format).

Related Services:

Recharge Rate Product
 $1.00  Nanodrop Service
Unit price per sample. RNA or dsDNA concentration determination using Nanodrop Spectrophotometer.
 $52.00  RNA 6000 Nano BioAnalyzer
Unit price per chip. Each RNA 6000 Nano chip can run up to 12 RNA or cDNA samples. Results include both a concentration estimate and a RNA Integrity Number (RIN).
 $220.00  Full Service Processing for GeneChips and Array Strips
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix cartridges and array strips only. Requires 300ng total RNA or more.
 $120.00  Full Service Processing for Array Plates
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix Array plates only. Requires 300ng total RNA or more.
 $310.00  Full Service Processing for GeneChips and Array Strips - Limited sample input
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix cartridges and array strips only. Requires 10ng total RNA or more.
 $190.00  Full Service Processing for Array Plates - Limited Sample input
Unit price per sample. Full service processing includes probe labeling from total RNA submitted, hybridization, scanning, and basic data analysis. This rate is for samples intended for use on Affymetrix Array plates only. Requires 10ng total RNA or more.
 $16.50  DNA isolation from Whole Blood
Unit price per sample. Genomic DNA can be isolated from up to 5mL of fresh whole blood. See link for additional options.