Whole Transcriptome Analysis
Global transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.
Currently, the most widely used method to analyze global patterns of gene expression is the DNA microarray. However, because microarrays are hybridization-based, you cannot use them to detect RNA transcripts not included in your array design or from repeated sequences. Also, microarrays offer limited dynamic range to detect subtle changes in expression level of target genes, which is critical in understanding biological response to stimuli or environmental changes.
SOLiD™ Whole Transcriptome Analysis Kit
Detect all known and novel RNAs in a transcriptome.
The sequencing-based SOLiD™ Whole Transcriptome Analysis Kit enables you to detect all known and novel RNAs present in biological samples, with no bias toward known RNA molecules as with probe-based technologies. The kit provides you with new views of a cell’s transcriptome, including:
- Expression of all coding and non-coding RNAs
- Identification of alternative splicing events
- Expressed SNPs (single nucleotide polymorphisms) or mutations
- Translocations and fusion transcripts
- Identify allele specific expression patterns
Together, the SOLiD™ Whole Transcriptome Analysis Kit and the ultra-high throughput SOLiD System:
- Conserve strandedness of cDNA, allowing you to discern between overlapping RNAs transcribed from the sense or antisense strand.
- Generate up to 600 million sequence reads per run for RNA expression analysis.
- Enable you to sequence up to 20 RNA libraries simultaneously (sample multiplexing), reducing the cost of analysis per sample.