Whereas much recent office design seeks to increase communication away from the private workspace, little is known about how the global structure of an office affects the location and amount of interaction. This study used syntax theories and methods to understand the effects of spatial layout on movement and copresence (i.e., visibility of people), and on face-to-face interaction. The study results showed that people preferred to interact more in individual workspaces than in semi-public and public territories of any office setting. The results also showed that co-presence was important for face-to-face interactions, and that more co-presence instigated more interactions. Surprisingly, movement did not consistently predict face-to-face interaction. This study suggests that while rigorous definitions of spatial layout can predict movement and interaction, these relationships are tempered by organizational programs.