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bulletFiber-Optic Roots

Our work with Near-Field Scanning Optical Microscopy led us to use pulled (tapered) fiber optic probes as intracellular sensors. The tips of these fibers were either coated with a layer of plasticized PVC


Artist's rendition of an optode inserted into a red blood cell

containing sensor materials, or they had a small bit of sensor material on the end which was photo-polymerized. These fiber-based optical nanosensors, called Optodes (as opposed to traditional, larger Electrodes) can be inserted into single cells to sense analyte levels. This technique works well, but even though the tip of the sensor may only be 100 nm in diameter, the fiber body quickly becomes larger up the taper and can create problems. Only two, or maybe three, sensors can be inserted at once into a large cell. The insertion can also damage the cell to the point that the cell's response may not be representative of its natural state.


Topics
Analytic Summary
Nano-Scale Analysis
Fiber-Optic Roots
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Lifetime Detection



 

 

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