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bulletLiposomal

The other commonly used method for PEBBLE delivery to cells is called liposomal delivery. This technique utilizes the natural system of some cell types to take in contents of liposomes which come into contact with their outer surface. The lipids in the micelles are very similar 
to the lipids in the cell walls, both having a hydrophyllic head and hydrophobic tails. This enables the two structures to merge and many things which are stuck to or contained within the liposomes end up in the cell cytoplasm. The fluorescence image shows the two colors of light from ratiometric calcium sensitive PEBBLEs. The green/yellow light from the Calcium Green dye indicates a wave of calcium sweeping across the cells, while the red light is from the Texas Red reference dye.


Schematic of liposomal delivery and cells which contain calcium PEBBLEs delivered by this method.


Topics
Analytic Summary
Nano-Scale Analysis
Fiber-Optic Roots
Enter PEBBLEs
Oxygen PEBBLE Example
Ratiometric Device
Response & Interference
Quantitative Imaging
Delivery Methods
Gene Gun
Liposomal
PEBBLE Production
Wet Chemistry Method
PEBBLE Sizes
Surface Modification
Decyl Methacrylate Example
Fabrication
Response
Nitric Oxide Sensing
cGMP Cycle
Lifetime Detection


 

 

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