 Liposomal
| The other commonly used method for PEBBLE
delivery to cells is called liposomal delivery. This
technique utilizes the natural system of some cell types
to take in contents of liposomes which come into contact
with their outer surface. The lipids in the micelles are
very similar |
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| to the lipids in the cell walls, both
having a hydrophyllic head and hydrophobic tails. This
enables the two structures to merge and many things
which are stuck to or contained within the liposomes end
up in the cell cytoplasm. The fluorescence image shows
the two colors of light from ratiometric calcium
sensitive PEBBLEs. The green/yellow light from the
Calcium Green dye indicates a wave of calcium sweeping
across the cells, while the red light is from the Texas
Red reference dye. |
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Schematic of liposomal delivery and cells which contain
calcium PEBBLEs delivered by this method.
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