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bulletQuantitative Imaging

Oxygen PEBBLEs have been used for ratiometric fluorescence imaging in C6 glioma cells. This figure shows a Nomarski (differential phase contrast) image (top - a) of the cultured cells on a coverslip. The next set of images are the green and red channels (b,c) of a confocal image showing the separate fluorescence from the reference dye and the oxygen sensing dye. Computer software has then be used (d,e) to superimpose the images, showing where signals are coming from within the cells. Changes in the red fluorescence can be used to show local changes in oxygen concentration, and ratio images can be generated showing oxygen concentration based on test-tube calibrations of the sensors.


Ratiometric Imaging with Oxygen PEBBLEs


Topics
Analytic Summary
Nano-Scale Analysis
Fiber-Optic Roots
Enter PEBBLEs
Oxygen PEBBLE Example
Ratiometric Device
Response & Interference
Quantitative Imaging
Delivery Methods
Gene Gun
Liposomal
PEBBLE Production
Wet Chemistry Method
PEBBLE Sizes
Surface Modification
Decyl Methacrylate Example
Fabrication
Response
Nitric Oxide Sensing
cGMP Cycle
Lifetime Detection



 

 

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