 Quantitative Imaging
| Oxygen PEBBLEs have been used for
ratiometric fluorescence imaging in C6 glioma cells.
This figure shows a Nomarski (differential phase
contrast) image (top - a) of the cultured cells on a
coverslip. The next set of images are the green and red
channels (b,c) of a confocal image showing the separate
fluorescence from the reference dye and the oxygen
sensing dye. Computer software has then be used (d,e) to
superimpose the images, showing where signals are coming
from within the cells. Changes in the red fluorescence
can be used to show local changes in oxygen
concentration, and ratio images can be generated showing
oxygen concentration based on test-tube calibrations of
the sensors. |
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Ratiometric Imaging with Oxygen PEBBLEs
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