As a first example of a PEBBLE sensor, we
take a ratiometric oxygen sensor. This has a Sol Gel
(silica glass which is formed at low temperatures in
solution) matrix and two different dyes embedded in its
core. The Ruthenium dye (orange) fluorescence is
quenched very efficiently in the presence of oxygen, so
its intensity goes down with more local oxygen
concentration. The Oregon Green dye (green) fluorescence
is not sensitive to oxygen, so its intensity stays the
same and can be used as a reference.
Ruthenium-DPP (Oxygen sensitive dye) |
Example of a ratiometric PEBBLE
using Ru-DPP sensing dye and Oregon Green as a reference
dye.
Oregon Green-488 (Molecular Probes)
|
Ratiometric Device 
