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bulletRatiometric Device

As a first example of a PEBBLE sensor, we take a ratiometric oxygen sensor. This has a Sol Gel (silica glass which is formed at low temperatures in solution) matrix and two different dyes embedded in its core. The Ruthenium dye (orange) fluorescence is quenched very efficiently in the presence of oxygen, so its intensity goes down with more local oxygen concentration. The Oregon Green dye (green) fluorescence is not sensitive to oxygen, so its intensity stays the same and can be used as a reference.


Ruthenium-DPP (Oxygen sensitive dye)


 Example of a ratiometric PEBBLE using Ru-DPP sensing dye and Oregon Green as a reference dye.





Oregon Green-488 (Molecular Probes)

This TEM (Transmission Electron Microscopy) image shows the size of some larger Sol Gel particles. On the next page, you can see how this ratiometric scheme is used.






Topics
Analytic Summary
Nano-Scale Analysis
Fiber-Optic Roots
Enter PEBBLEs
Oxygen PEBBLE Example
Ratiometric Device
Response & Interference
Quantitative Imaging
Delivery Methods
Gene Gun
Liposomal
PEBBLE Production
Wet Chemistry Method
PEBBLE Sizes
Surface Modification
Decyl Methacrylate Example
Fabrication
Response
Nitric Oxide Sensing
cGMP Cycle
Lifetime Detection


 

 

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