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Example of a ratiometric PEBBLE using Ru-DPP sensing dye and Oregon Green as a reference dye.
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Ruthenium-DPP (Oxygen sensitive dye)
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Oregon Green-488 (Molecular Probes)
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As a first example of a PEBBLE sensor, we take a ratiometric oxygen sensor. This has a Sol Gel (silica glass which is formed at low temperatures in solution) matrix and two different dyes embedded in its core. The Ruthenium dye (orange) fluorescence is quenched very efficiently in the presence of oxygen, so its intensity goes down with more local oxygen concentration. The Oregon Green dye (green) fluorescence is not sensitive to oxygen, so its intensity stays the same and can be used as a reference.
TEM micrograph of Sol Gel nanospheres.
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This TEM (Transmission Electron Microscopy) image shows the size of some larger Sol Gel particles. On the next page, you can see how this ratiometric scheme is used.
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