RAMAMOORTHY LABORATORY - Biological solid-state NMR spectroscopy
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Recent publications

Designations in first column: AMP: publication dealing with antimicrobial peptides, Prot: membrane proteins and peptides, NMR: solid-state method development, LBL: lipid bilayers, LC: liquid crystals, PO: Polymers.

2008
AMP   Porcelli F, Verardi R, Shi L, Henzler-Wildman KA, Ramamoorthy A, Veglia G. NMR Structure of the Cathelicidin-Derived Human Antimicrobial Peptide LL-37 in Dodecylphosphocholine Micelles. Biochemistry, in press.
NMR   Ramamoorthy A, Lee DK, Santos JS, Henzler-Wildman KA. Nitrogen-14 Solid-State NMR Spectroscopy of Aligned Phospholipid Bilayers to Probe Peptide-Lipid Interaction and Oligomerization of Membrane Associated Peptides. J. Am. Chem. Soc., in press.
AMP   Gottler LM, Lee HY, Shelburne CE, Ramamoorthy A, Marsh ENG. Using Fluorous Amino Acids to Modulate the Biological Activity of an Antimicrobial Peptide. ChemBioChem 9 (2008) 370-373.
Prot   Brender JR, Lee EL, Cavitt MA, Gafni A, Steel DG, Ramamoorthy A. Amyloid Fiber Formation and Membrane Disruption are Separate Processes Localized in Two Distinct Regions of the Amylin Peptide. J. Am. Chem. Soc., in press.
NMR   Xu J, Struppe J, Ramamoorthy A. Two-dimensional homonuclear chemical shift correlation established by the cross-relaxation driven spin diffusion in solids. J. Chem. Phys. 128 (2008) 052308.
AMP   Hoskin DW, Ramamoorthy A. Studies on anticancer activities of antimicrobial peptides. Biochim. Biophys. Acta 1778 (2008) 357-375.
2007
Prot   Brender, JR, Dürr UHN, Heyl D, Budarapu MB, Ramamoorthy A. Membrane Fragmentation by an Amyloidogenic Fragment of Human Islet Amyloid Polypeptide Detected by Solid-State NMR Spectroscopy of Membrane Nanotubes. Biochim. Biophys. Acta 1768 (2007) 2026-2029.
PO   Podsiadlo P, Kaushik AK, Arruda EM, Waas AM, Sup B, Shim, Xu J, Nandivada H, Pumplin BG, Lahann J, Ramamoorthy A, Kotov NA, Ultrastrong and Stiff Layered Polymer Nanocomposites, Science. 318 (2007) 80-83.
Prot   Dürr UHN, Yamamoto K, Im SC, Waskell L, Ramamoorthy A, Solid-State NMR Reveals Structural and Dynamical Properties of a Membrane-Anchored Electron-Carrier Protein, Cytochrome b(5), J. Am. Chem. Soc. 129 (2007) 6670-6671.
Prot   Dürr UHN, Waskell L, Ramamoorthy A. The cytochromes P450 and b5 and their reductases-Promising targets for structural studies by advanced solid-state NMR spectroscopy, Biochim. Biophys. Acta. 1768 (2007) 3235-3259.
LC  

Textbook: A. Ramamoorthy (Ed.), Thermotropic Liquid Crystals: Recent Advances, Springer, Dordrecht, The Netherlands 2007

 
AMP   Shelburne C, An F, Dhople V, Ramamoorthy A, Lopatin D, Lantz M, The spectrum of anti-microbial activity of the bacteriocin Subtilosin A, J. Antimicrob. Chemother., 59 (2007) 297-300.
Prot   Ramamoorthy A, Kandasamy SK, Lee DK, Kidambi S, Larson RG, Structure, Topology and tilt of cell-signaling peptides containing nuclear localization sequences in membrane bilayers determined by solid-state NMR and molecular dynamics simulation studies, Biochemistry 46 (2007) 965-975.
LBL   Dvinskikh SV, Dürr UHN, Yamamoto K, Ramamoorthy A, High-resolution 2D NMR spectroscopy of bicelles to measure the membrane interaction of ligands, J. Am. Chem. Soc. 129 (2007) 794-802.
LBL   Dvinskikh SV, Yamamoto K, Dürr UHN, Ramamoorthy A, Sensitivity and resolution enhancement in solid-state NMR spectroscopy of bicelles, J. Magn. Reson. 184 (2007) 228-235.
2006
NMR   Fujiwara T, Ramamoorthy A, How far can the sensitivity of NMR be
increased?, Ann. Rep. NMR Spectrosc. 58 (2006) 155-175.
AMP   Ramamoorthy A, Thennarasu S, Tan A, Gottipati K, Sreekumar S, Heyl DL, An FYP, Shelburne CE, Deletion of all cycteines in Tachyplesin I abolishes hemolytic activity and retains antimicrobial activity and LPS selective binding, Biochemistry 45 (2006) 6529-6540.
AMP   Ramamoorthy A, Thennarasu S, Lee DK, Tan A, Maloy L, Solid-State NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from Magainin 2 and Melittin, Biophys. J . 91 (2006) 206-216.
AMP  

Porcelli F, Buck-Koehntop B, Thennarasu S, Ramamoorthy A, Veglia G, Structures of the dimeric and monomeric variants of magainin antimicrobial peptides (MSI-78 and MSI-594) in micelles and bilayers by NMR spectroscopy, Biochemistry 45 (2006) 5793-5799.
AMP   Epand RF, Ramamoorthy A, Epand RM, Membrane lipid composition and the interaction of pardaxin: The role of cholesterol, Prot. Pept. Lett. 13 (2006) 1-5.
AMP   Ramamoorthy A, Thennarasu S, Tan A, Lee DK, Clayberger C, Krensky AM, Cell selectivity correlates with membrane-specific interactions: A case study on the antimicrobial peptide G15 derived from granulysin, Biochim. Biophys. Acta 1758 (2006) 154-163.
AMP

Special issue: A. Ramamoorthy (Ed.), Membrane biophysics of antimicrobial peptides, Biochim. Biophys. Acta 1758 (2006) 1177-1540.

 
AMP Moon JY, Henzler-Wildman KA, Ramamoorthy A, Expression and purification of a recombinant LL-37 from Escherichia coli, Biochim. Biophys. Acta 1758 (2006) 1351-1358.
AMP Dürr UHN, Sudheendra US, Ramamoorthy A, LL-37, the only human member of the cathelicidin family of antimicrobial peptides, Biochim. Biophys. Acta 1758 (2006) 1408-1425.
AMP   Dhople V, Krukemeyer A, Ramamoorthy A, The human beta-defensin-3, an antibacterial peptide with multiple biological functions, Biochim. Biophys. Acta 1758 (2006) 1499-1512.
NMR

Textbook: A. Ramamoorthy (Ed.), NMR Spectroscopy of Biological Solids, Taylor & Francis, New York 2006.

 
NMR Saalwächter K, Ramamoorthy A, Sensitivity enhancement by inverse detection in solids, NMR Spectroscopy of Biological Solids, Taylor and Francis Group, New York (2006) 151-175.
LBL   Dvinskikh SV, Durr U, Yamamoto K, Ramamoorthy A, A high-resolution solid-state NMR approach for the structural studies of bicelles, J. Am. Chem. Soc. 128 (2006) 6326-7.
NMR

Dvinskikh SV, Yamamoto K, Ramamoorthy A, Heteronuclear isotropic mixing separated local field NMR spectroscopy, J. Chem. Phys. 125 (2006) 034507.
NMR

Yamamoto K, Dvinskikh SV, Ramamoorthy A, Measurement of heteronuclear dipolar couplings using a rotating frame solid-state NMR experiment, Chem. Phys. Lett. 419 (2006) 533-536.
NMR

Dvinskikh SV, Yamamoto K, Ramamoorthy A, Separated local field NMR spectroscopy by windowless isotropic mixing, Chem. Phys. Lett. 419 (2006) 168-173.
2005
Prot

Zand R, Pointer-Keenan CD, Lee DK, Hallock K, Tan A, Ramamoorthy A, A solid state NMR study of MBP interaction with lipid bilayers, J. Neurochem. 94 (Suppl. 1, 2005) 51.
AMP Thennarasu S, Lee DK, Tan A, Kari UP, Ramamoorthy A, Antimicrobial activity and membrane selective interactions of a synthetic lipopeptide MSI-843, Biochim. Biophys. Acta 1711 (2005) 49-58.
AMP

Thennarasu S, Lee DK, Poon A, Kawulka KE, Vederas JC, Ramamoorthy A, Membrane permeabilization, orientation, and antimicrobial mechanism of subtilosin A, Chem. Phys. Lipids 137 (2005) 38-51.
AMP

Powers JPS, Tan A, Ramamoorthy A, Hancock REW, Solution structure and interaction of the antimicrobial polyphemusins with lipid membranes, Biochemistry 44 (2005) 15504-15513.
AMP

Heyl DL, Sreekumar S, Gottipati K, Thennarasu S, Ramamoorthy A, Design, synthesis, and analysis of a cysteine-deleted analog of the antimicrobial peptide Tachyplesin-I, Biopolymers 80 (2005) 541-541.
NMR

Yamamoto K, Lee DK, Ramamoorthy A, Broadband-PISEMA solid-state NMR spectroscopy, Chem. Phys. Lett. 407(2005) 289-293.
NMR

Yamamoto K, Ermakov VL, Lee DK, Ramamoorthy A, PITANSEMA-MAS, a solid-state NMR method to measure heteronuclear dipolar couplings under MAS, Chem. Phys. Lett. 408 (2005) 118-122.
LBL Mecke A, Lee DK, Ramamoorthy A, Orr BG, Holl MMB, Membrane thinning due to antimicrobial peptide binding: An atomic force microscopy study of MSI-78 in lipid bilayers, Biophys. J. 99 (2005) 4043-4050.
LBL

Mecke A, Lee DK, Ramamoorthy A, Orr BG, Holl MMB, Synthetic and natural polycationic polymer nanoparticles interact selectively with fluid-phase domains of DMPC lipid bilayers, Langmuir 21 (2005) 8588-8590.
LC Narasimhaswamy T, Monette M, Lee DK, Ramamoorthy A, Solid-state NMR characterization and determination of the orientational order of a nematogen, J. Phys. Chem. B109 (2005) 19696-19703.
LC

Narasimhaswamy T, Lee DK, Somanathan N, Ramamoorthy A, Solid-state NMR characterization of a novel thiophene-based three phenyl ring mesogen, Chem. Materials 17 (2005) 4567-4569.
LC

Narasimhaswamy T, Lee DK, Yamamoto K, Somanathan N, Ramamoorthy A, A 2D solid-state NMR experiment to resolve overlapping aromatic resonances of thiophene-based nematogens, J. Am. Chem. Soc. 127 (2005) 6958-6959.
LC

Narasimhaswamy T, Somanathan N, Lee DK, Ramamoorthy A, Synthesis and C-13 CPMAS NMR characterization of novel thiophene-based nematogens, Chem. Materials 17 (2005) 2013-2018.
2004
Prot

Pointer-Keenan CD, Lee DK, Hallock K, Tan AM, Zand R, Ramamoorthy A, Investigation of the interaction of myelin basic protein with phospholipid bilayers using solid-state NMR spectroscopy, Chem. Phys. Lip. 132 (2004) 47-54.
AMP

Porcelli F, Buck B, Lee DK, Hallock KJ, Ramamoorthy A, Veglia G, Structure and orientation of pardaxin determined by NMR experiments in model membranes, J. Biol. Chem. 279 (2004) 45815-45823.
AMP

Henzler-Wildman KA, Martinez GV, Brown MF, Ramamoorthy A, Perturbation of the hydrophobic core of lipid bilayers by the human antimicrobial peptide LL-37, Biochemistry 43 (2004) 8459-8469.
AMP

Ramamoorthy A, Lee DK, Wildman KH, Membrane disrupting mechanism of LL-37 and magainin analogs, Biophys. J. 86 (Part 2 Suppl. 2004) 41A.
LBL

Mecke A, Uppuluri S, Sassanella TM, Lee DK, Ramamoorthy A, Baker JR, Orr BG, Holl MMB, Direct observation of lipid bilayer disruption by poly(amidoamine) dendrimers, Chem. Phys. Lip. 132 (2004) 3-14.
LBL

Lee DK, Wildman KH, Ramamoorthy A, Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures, J. Am. Chem. Soc. 126 (2004) 2318-2319.
LBL

Santos JS, Lee DK, Ramamoorthy A, Effects of antidepressants on the conformation of phospholipid headgroups studied by solid- state NMR, Magn. Reson. Chem. 42 (2004) 105-114.
NMR

Ramamoorthy A, Wei YF, Lee DK, PISEMA solid-state NMR spectroscopy, Ann. Rep. NMR Spectrosc. 52 (2004) 1-52.
NMR

Lee DK, Narasimhaswamy T, Ramamoorthy A, PITANSEMA, a low-power PISEMA solid-state NMR experiment, Chem. Phys. Lett. 399 (2004) 359-362.
NMR

Wildman KAH, Ramamoorthy A, Wakamiya T, Yoshikawa T, Crisma M, Toniolo C, Formaggio F, A study of a C-alpha,C-beta-didehydroalanine homo-oligopeptide series in the solid-state by C-13 cross-polarization magic angle spinning NMR, J. Pept. Sci. 10 (2004) 336-341.
NMR

Poon A, Birn J, Ramamoorthy A, How does an amide-N-15 chemical shift tensor vary in peptides?, J. Phys. Chem. B 108 (2004) 16577-16585.
NMR

Bim J, Poon A, Mao Y, Ramamoorthy A, Ab initio study of C-13 alpha chemical shift anisotropy tensors in peptides, J. Am. Chem. Soc. 126 (2004) 8529-8534.
NMR Birn J, Poon A, Ramamoorthy A, Variation of alpha-carbon-13 and amide-nitrogen-15 chemical shift tensors in polypeptides, Biophys. J. 86 (Part 2 Suppl. 2004) 73A.
2003
AMP Wildman KAH, Lee DK, Ramamoorthy A, Mechanism of lipid bilayer disruption by the human antimicrobial peptide, LL-37, Biochemistry 42 (2003) 6545.
AMP Hallock KJ, Lee DK, Ramamoorthy A, MSI-78, an analogue of the magainin antimicrobial peptides, disrupts lipid bilayer structure via positive curvature strain, Biophys. J. 84 (2003) 3052.
LBL Mascioni A, Porcelli F, Ilangovan U, Ramamoorthy A, Veglia G, Conformational preferences of the amylin nucleation site in SDS micelles: An NMR study, Biopolymers 69 (2003) 29.
NMR Wildman KAH, Wilson EE, Lee DK, Ramamoorthy A, Determination of the conformation and stability of simple homopolypeptides using solid-state NMR, Solid State Nucl. Magn. Reson. 24 (2003) 94.
NMR Kidambi S, Schurko RW, Hung I, Ramamoorthy A, Nuclear magnetic resonance studies of metals in solid state non-metallic materials, Mater. Sci. Tech. 19 (2003) 1191-1196.
NMR Kidambi S, Lee DK, Ramamoorthy A, Interaction of Cd and Zn with biologically important ligands characterized using solid-state NMR and ab initio calculations, Inorg. Chem. 42 (2003) 3142.
NMR Kidambi S, Lee DK, Ramamoorthy A, Characterization of metal centers in bioinorganic complexes using ab initio calculations of Cd-113 chemical shifts, Inorg. Chem. 42 (2003) 2200.
2002
AMP Hallock KJ, Lee DK, Omnaas J, Mosberg HI, Ramamoorthy A, Membrane composition determines pardaxin's mechanism of lipid bilayer disruption, Biophys. J. 83 (2002) 1004-1013.
LBL Hallock KJ, Henzler Wildman KA, Lee DK, Ramamoorthy A, Sublimable solids can be used to mechanically align lipid bilayers for solid-state NMR studies, Biophys. J. 82 (2002) 2499.
LBL Santos JS, Lee DK, Hallock KJ, Ramamoorthy A, Cholesterol and antidepressant drug interactions with phospholipids - A solid-state NMR study, Recent Research Developments in Physical Chemistry, Transworld Research Network 6 (2002) 179-211.
NMR Henzler Wildman KA, Lee DK, Ramamoorthy A, Determination of effect of a-helix and b-sheet stability in the solid-state: A solid-state NMR investigation of poly(L-alanine), Biopolymers 64 (2002) 246-254.
NMR Shekar SC, Lee DK, Ramamoorthy A, Chemical shift anisotropy and offset effects in cross polarization solid-state NMR spectroscopy, J. Magn. Reson. 157 (2002) 223-234.
NMR Wei Y, Lee DK, McDermott AE, Ramamoorthy A, A 2D MAS solid-state NMR method to recover the amplified heteronuclear dipolar and chemical shift anisotropic interactions, J. Magn. Reson. 158 (2002) 23-35.
NMR Shekar SC, Ramamoorthy A, Wittebort RJ, Determination of the chemical shielding orientation from two or one of the three conventional rotations of a single crystal, J. Magn. Reson. 155 (2002) 257-262.
NMR Wei YF, Lee DK, Hallock KJ, Ramamoorthy A, One-dimensional 1H-detected solid-state NMR experiment to determine amide-1H chemical shifts in peptides, Chem. Phys. Lett. 351 (2002) 42-46.
NMR Taylor DM, Ramamoorthy A, Coherence transfer through homonuclear dipolar coupling in an unoriented two spin-1/2 solid-state system, J. Mol. Struct. 602-603 (2002) 115-124.
NMR

Kidambi S, Ramamoorthy A, Quantum chemical calculations of cadmium chemical shifts in inorganic complexes, J. Phys. Chem. A 106 (2002) 10363.
2001
NMR Wei Y, Lee DK, Ramamoorthy A, Solid-state 13C chemical shift anisotropy tensors of polypeptides, J. Am. Chem. Soc. 123 (2001) 6118-6126.
NMR Wei Y, Lee DK, Ramamoorthy A, A two-dimensional magic angle decoupling and magic angle turning solid-state NMR method - An application to study chemical shift tensors from peptides that are non-selectively labeled with 15N isotope, J. Phys. Chem. B 104 (2001) 4752-4762.
NMR Shekar SC, Lee DK, Ramamoorthy A, An experimental strategy to dramatically reduce the RF power used in cross polarization solid-state NMR spectroscopy, J. Am. Chem. Soc. 123 (2001) 7467-7468.
NMR Wei YF, Ramamoorthy A, 2D 15N-15N isotropic chemical shift correlation established by 1H-1H dipolar coherence transfer in biological solids, Chem. Phys. Lett. 342 (2001) 312-316.
NMR Brender JR, Taylor DM, Ramamoorthy A, Orientation of amide-nitrogen-15 chemical shift tensors in peptides: A quantum chemical study, J. Am. Chem. Soc . 123 (2001) 914-922.
NMR

Shekar SC, Ramamoorthy A, The unitary evolution operator for cross-polarization schemes in NMR, Chem. Phys. Lett. 342 (2001) 127-134.

2000
NMR Hallock KJ, Lee DK, Ramamoorthy A, The effects of librations on the 13C chemical shift and 2H electric field gradient tensors in b-calcium formate, J. Chem. Phys. 113 (2000) 11187.
NMR Wei YF, Lee DK, Ramamoorthy A, One-dimensional dipolar-shift spectroscopy under magic angle spinning to determine the chemical-shift anisotropy tensors, Chem. Phys. Lett. 324 (2000) 20.
NMR

Stout KL, Hallock KJ, Kampf JW, Ramamoorthy A, N-Acetyl-L-phenylalanine, Acta Cryst. C56 (2000) e100.
1999
NMR   Taylor DM, Ramamoorthy A, Analysis of dipolar-coupling mediated coherence transfer in a homonuclear two spin 1/2 solid-state system, J. Magn. Reson. 141 (1999) 18.
NMR   Lee DK, Santos JS, Ramamoorthy A, Application of one-dimensional dipolar-shift solid-state NMR spectroscopy to study the backbone conformation of membrane-associated peptides in phospholipid bilayers, J. Phys. Chem. B 103 (1999) 8383.
NMR   Lee DK, Santos JS, Ramamoorthy A, Nitrogen-15 chemical shift anisotropy and 1H-15N dipolar coupling tensors associated with the phenylalanine residue in the solid-state, Chem. Phys. Lett. 309(1999) 209.
NMR   Ramamoorthy A, Wu CH, Opella SJ, Experimental aspects of multidimensional solid-state NMR correlation spectroscopy, J. Magn. Reson. 140 (1999) 131.
NMR   Lee DK, Ramamoorthy A, Determination of the solid-state conformations of polyalanine using magic-angle spinning NMR spectroscopy, J. Phys. Chem. B 103 (1999) 271.
NMR  

Hallock KJ, Lee DK, Ramamoorthy A, Carbon-13 chemical shifts and 1H-13C dipolar couplings in metal sandwich solids using one-dimensional solid-state NMR spectroscopy, Chem. Phys. Lett. 302 (1999) 175.

 

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