Here is the sequence of the CMV Construct that is used in this lab. It contains the rat m Opiate Receptor (rMOR) plus some additional 5' and 3' flanking Sequence as an insert. The amino acid 1-letter code is given below its codon.

 

This sequence is also known as CMV-m4B, or simply m4B

Regions of interest are:
1. 276-680 = The Cytomegalovirus (CMV) promoter
(All but the two 5'-most nucleotides identified originally as the promoter sequence). The original CMV construct is maintained through nucleotide 894, and includes the CAAT box (CAAAT, from 736-740), the TATA box (TATAA, from 771-775), and the transcription start site (T, 798).
2. 3634-4494 = Ampicillin Resistance Gene (AMPr). This begins with the start codon (ATG, 3634-3636), and ends with the stop codon (TAA, 4492-4494).
3. 1135-2333 = The rat Mu Opiate Receptor (rMOR) gene.
The entire sequence is as follows:


 
        10        20        30        40        50         60        70        80        90       100 GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAAC CGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAATTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTG

 
       110       120       130       140       150       160  & nbsp    170       180       190       200 GCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTC CGTTAATTACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATACAACACACCTTAACACTCGCCTATTGTTAAAG

 
       210       220       230       240       250       260  & nbsp    270       280       290       300 ACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGGCTGCAGGTCGACTCTAGAGGATCCGGCTAGAGTCCGTTACATAACTTACGGTAAATGGC TGTGTCCTTTGTCGATACTGGTACTAATGCGGTTCGAACCCGACGTCCAGCTGAGATCTCCTAGGCCGATCTCAGGCAATGTATTGAATGCCATTTACCG

 
       310       320       330       340       350       360  & nbsp    370       380       390       400 CCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGG GGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCC

 
       410       420       430       440       450       460  & nbsp    470       480       490       500 TGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTG ACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGAC

 
       510       520       530       540       550       560  & nbsp    570       580       590       600 GCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTAC CGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATG

 
       610       620       630       640       650       660  & nbsp    670       680       690       700 ATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACT TAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGA

 
       710       720       730       740       750       760  & nbsp    770       780       790       800 TTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA AAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCACTTGGCAGT

 
       810       820       830       840       850       860  & nbsp    870       880       890       900 GATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCGGGAACGGTGCATTGGAACGGACCTG CTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTGTGGCCCTGGCTAGGTCGGAGGCGCCGGCCCTTGCCACGTAACCTTGCCTGGAC

 
       910       920       930       940       950       960  & nbsp    970       980       990      1000 CAGCCCAAGCTGAAGATCCGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATCGGCACGAGCTTCTGCCTGCCGCTCTTCTCTGGTTC GTCGGGTTCGACTTCTAGGCCATGGCCCGGGGGGGAGCTCCAGCTGCCATAGCTATTCGAACTAGCCGTGCTCGAAGACGGACGGCGAGAAGAGACCAAG                     KpnI site=start of the rMOR insert

 
      1010      1020      1030      1040      1050      1060      1070& nbsp     1080      1090      1100 CACTAGGGCTGGTCCATGTAAGAATCTGACGGAGCCTAGGGCAGCTGTGAGAGGAAGAGGCTGGGGCGCGTGGAACCCGAAAAGTCTGAGTGCTCTCAGT GTGATCCCGACCAGGTACATTCTTAGACTGCCTCGGATCCCGTCGACACTCTCCTTCTCCGACCCCGCGCACCTTGGGCTTTTCAGACTCACGAGAGTCA

 
      1110      1120      1130      1140      1150      1160      1170& nbsp     1180      1190      1200 TACAGCCTACCTAGTCCGCAGCAGGCCTTCAGCACCATGGACAGCAGCACCGGCCCAGGGAACACCAGCGACTGCTCAGACCCCTTAGCTCAGGCAAGTT ATGTCGGATGGATCAGGCGTCGTCCGGAAGTCGTGGTACCTGTCGTCGTGGCCGGGTCCCTTGTGGTCGCTGACGAGTCTGGGGAATCGAGTCCGTTCAA
                   &n bsp                 M  D  S  S  T  G  P  G  N  T  S  D  C  S  D  P& nbsp L  A  Q  A  S


 
      1210      1220      1230      1240      1250      1260      1270& nbsp     1280      1290      1300 GCTCCCCAGCACCTGGCTCCTGGCTCAACTTGTCCCACGTTGATGGCAACCAGTCCGATCCATGCGGTCTGAACCGCACCGGGCTTGGCGGGAACGACAG CGAGGGGTCGTGGACCGAGGACCGAGTTGAACAGGGTGCAACTACCGTTGGTCAGGCTAGGTACGCCAGACTTGGCGTGGCCCGAACCGCCCTTGCTGTC C  S  P  A  P  G  S  W  L  N  L  S  H  V  D  G  N  Q  S  D  P  C  G  L& nbsp N  R  T  G  L  G  G  N  D  S

 
      1310      1320      1330      1340      1350      1360      1370& nbsp     1380      1390      1400 CCTGTGCCCTCAGACCGGCAGCCCTTCCATGGTCACAGCCATTACCATCATGGCCCTCTACTCTATCGTGTGTGTAGTGGGCCTCTTCGGAAACTTCCTG GGACACGGGAGTCTGGCCGTCGGGAAGGTACCAGTGTCGGTAATGGTAGTACCGGGAGATGAGATAGCACACACATCACCCGGAGAAGCCTTTGAAGGAC   L  C  P  Q  T  G  S  P  S  M  V  T  A  I  T  I  M  A  L  Y  S  I  V&n bsp C  V  V  G  L  F  G  N  F  L

 
      1410      1420      1430      1440      1450      1460      1470& nbsp     1480      1490      1500 GTCATGTATGTGATTGTAAGATACACCAAAATGAAGACTGCCACCAACATCTACATTTTCAACCTTGCTCTGGCAGACGCCTTAGCGACCAGTACACTGC CAGTACATACACTAACATTCTATGTGGTTTTACTTCTGACGGTGGTTGTAGATGTAAAAGTTGGAACGAGACCGTCTGCGGAATCGCTGGTCATGTGACG  V  M  Y  V  I  V  R  Y  T  K  M  K  T  A  T  N  I  Y  I  F  N  L  A &n bspL  A  D  A  L  A  T  S  T  L

 
      1510      1520      1530      1540      1550      1560      1570& nbsp     1580      1590      1600 CCTTTCAGAGTGTCAACTACCTGATGGGAACATGGCCCTTCGGAACCATCCTCTGCAAGATCGTGATCTCAATAGATTACTACAACATGTTCACCAGCAT GGAAAGTCTCACAGTTGATGGACTACCCTTGTACCGGGAAGCCTTGGTAGGAGACGTTCTAGCACTAGAGTTATCTAATGATGTTGTACAAGTGGTCGTA P  F  G  S  V  N  Y  L  M  G  T  W  P  F  G  T  I  L  C  K  I  V  I  S& nbsp I  D  Y  Y  N  M  F  T  S  I

 
      1610      1620      1630      1640      1650      1660      1670& nbsp     1680      1690      1700 ATTCACCCTCTGCACCATGAGCGTGGACCGCTACATTGCTGTCTGCCACCCAGTCAAAGCCCTGGATTTCCGTAAACCFFGAAATGCCAAAATCGTCAAC TAAGTGGGAGACGTGGTACTCGCACCTGGCGATGTAACGACAGACGGTGGGTCAGTTTCGGGACCTAAAGGCATGGGGGGCTTTACGGTTTTAGCAGTTG   F  T  L  C  T  M  S  V  D  R  Y  I  A  V  C  H  P  V  K  A  L  D  F&n bsp R  T  P  R  N  A  K  I  V  N

 
      1710      1720      1730      1740      1750      1760      1770& nbsp     1780      1790      1800 GTCTGCAACTGGATCCTCTCTTCTGCCATCGGTCTGCCTGTAATGTTCATGGCAACCACAAAATACAGGCAGGGGTCCATAGATTGCACCCTCACGTTCT CAGACGTTGACCTAGGAGAGAAGACGGTAGCCAGACGGACATTACAAGTACCGTTGGTGTTTTATGTCCGTCCCCAGGTATCTAACGTGGGAGTGCAAGA  V  C  N  W  I  L  S  S  A  I  G  L  P  V  M  F  M  A  T  T  K  Y  R &n bspQ  G  S  I  D  C  T  L  T  F

 
      1810      1820      1830      1840      1850      1860      1870& nbsp     1880      1890      1900 CCCACCCAACCTGGTACTGGGAGAACCTGCTCAAAATCTGTGTCTTTATCTTCGCTTTCATCATGCCGGTCCTCATCATCACTGTGTGTTACGGCCTGAT GGGTGGGTTGGACCATGACCCTCTTGGACGAGTTTTAGACACAGAAATAGAAGCGAAAGTAGTACGGCCAGGAGTAGTAGTGACACACAATGCCGGACTA S  H  P  T  W  Y  W  E  N  L  L  K  I  C  V  F  I  F  A  F  I  M  P  V& nbsp L  I  I  T  V  C  Y  G  L  M

 
      1910      1920      1930      1940      1950      1960      1970& nbsp     1980      1990      2000 GATCTTACGACTCAAGAGCGTTCGCATGCTATCGGGCTCCAAAGAAAAGGACAGGAATCTGCGCAGGATCACCCGGATGGTGCTGGTGGTCGTGGCTGTA CTAGAATGCTGAGTTCTCGCAAGCGTACGATAGCCCGAGGTTTCTTTTCCTGTCCTTAGACGCGTCCTAGTGGGCCTACCACGACCACCAGCACCGACAT   I  L  R  L  K  S  V  R  M  L  S  G  S  K  E  K  D  R  N  L  R  R  I&n bsp T  R  M  V  L  V  V  V  A  V

 
      2010      2020      2030      2040      2050      2060      2070& nbsp     2080      2090      2100 TTTATCGTCTGCTGGACCCCCATCCACATCTACGTCATCATCAAAGCGCTGATCACGATTCCAGAAACCACATTTCAGACGGTTTCCTGGCACTTCTGCA AAATAGCAGACGACCTGGGGGTAGGTGTAGATGCAGTAGTAGTTTCGCGACTAGTGCTAAGGTCTTTGGTGTAAAGTCTGCCAAAGGACCGTGAAGACGT  F  I  V  C  W  T  P  I  H  I  Y  V  I  I  K  A  L  I  T  I  P  E  T &n bspT  F  N  T  V  S  W  H  F  C

 
      2110      2120      2130      2140      2150      2160      2170& nbsp     2180      2190      2200 TTGCTTTGGGTTACACGAACAGCTGCCTGAATCCAGTTCTTTACGCCTTCCTGGATGAAAACTTCAAGCGATGCTTCAGAGAGTTCTGCATCCCAACCTC AACGAAACCCAATGTGCTTGTCGACGGACTTAGGTCAAGAAATGCGGAAGGACCTACTTTTGAAGTTCGCTACGAAGTCTCTCAAGACGTAGGGTTGGAG I  A  L  G  Y  T  N  S  C  L  N  P  V  L  Y  A  F  L  D  E  N  F  K  R& nbsp C  F  R  E  F  C  I  P  T  S

 
      2210      2220      2230      2240      2250      2260      2270& nbsp     2280      2290      2300 GTCCACGATCGAACAGCAAAACTCCACTCGAGTCCGTCAGAACACTAGGGAACATCCCTCCACGGCTAATACAGTGGATCGAACTAACCACCAGCTAGAA CAGGTGCTAGCTTGTCGTTTTGAGGTGAGCTCAGGCAGTCTTGTGATCCCTTGTAGGGAGGTGCCGATTATGTCACCTAGCTTGATTGGTGGTCGATCTT   S  T  I  E  Q  Q  N  S  T  R  V  R  Q  N  T  R  E  H  P  S  T  A  N&n bsp T  V  D  R  T  N  H  Q  L  E

 
      2310      2320      2330      2340      2350      2360      2370& nbsp     2380      2390      2400 AATCTGGAGGCAGAAACTGCTCCATTGCCCTAACTGGGTCTCACACCATCCAGACCCTCGCTAATCGAATTCCTGCAGCCCGGGGGATCCACTAGTGCGG TTAGACCTCCGTCTTTGACGAGGTAACGGGATTGACCCAGAGTGTGGTAGGTCTGGGAGCGATTAGCTTAAGGACGTCGGGCCCCCTAGGTGATCACGCC
 N  L  E  A  E  T  A  P  L  P END                                        end of rMOR insert=SpeI site

 
      2410      2420      2430      2440      2450      2460      2470       2480      2490      2500 CCGCAGATCTGGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATT GGCGTCTAGACCCCACCGTAGGGACACTGGGGAGGGGTCACGGAGAGGACCGGGACCTTCAACGGTGAGGTCACGGGTGGTCGGAACAGGATTATTTTAA

 
      2500      2520      2530      2540      2550      2560      2570& nbsp     2580      2590      2600 AAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCCCAAGTTGGGAAGACAACCTGTA TTCAACGTAGTAAAACAGACTGATCCACAGGAAGATATTATAATACCCCACCTAACCFFACCATACCTCGTTCCCCGGGTTCAACCCTTCTGTTGGACAT

 
      2610      2620      2630      2640      2650      2660      2670& nbsp     2680      2690      2700 GGGCCTGCGGGGTCTATTCGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTC CCCGGACGCCCCAGATAAGCCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTTAGAGGCGGAGGACCCAAGTTCGCTAAGAGGACGGAG

 
      2710      2720      2730      2740      2750      2760      2770& nbsp     2780      2790     2800  AGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT TCGGAGGGCTCAACAACCCTAAGGTCCGTACGTACTGGTCCGAGTCGATTAAAAACAAAAAAACCATCTCTGCCCCAAAGTGGTATAACCGGTCCGACCA

 
      2810      2820      2830      2840      2850      2860      2870& nbsp     2880      2890      2900 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTA GAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGTTTAACGACCCTAATGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGACTAAAAT

 
      2910      2920      2930      2940      2950      2960      2970& nbsp     2980      2990      3000 AAATAACTATACCAGCAGGAGGACGTCCAGACACAGCATAGGCTACCTGCCATGGCCCAACCGGTGGGACATTTGAGTTGCTTGCTTGGCACTGTCCTCT TTTATTGATATGGTCGTCCTCCTGCAGGTCTGTGTCGTATCCGATGGACGGTACCGGGTTGGCCACCCTGTAAACTCAACGAACGAACCGTGACAGGAGA

 
      3010      3020      3030      3040      3050      3060      3070& nbsp     3080      3090      3100 CATGCGTTGGGTCCACTCAGTAGATGCCTGTTGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC GTACGCAACCCAGGTGAGTCATCTACGGACAACTTAAGTGACCGGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGG

 
      3110      3120      3130      3140      3150      3160      3170& nbsp     3180      3190      3200 TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCT AACGTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGGA

 
      3210      3220      3230      3240      3250      3260      3270& nbsp     3280      3290      3300 GATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCC CTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGG

 
      3310      3320      3330      3340      3350      3360      3370& nbsp     3380      3390      3400 CGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTC GCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAG

 
      3410      3420      3430      3440      3450      3460      3470& nbsp     3480      3490      3500 AGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGAC TCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAAGAATCTG

 
      3510      3520      3530      3540      3550      3560      3570& nbsp     3580      3590      3600 GTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA CAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACT

 
      3610      3620      3630      3640      3650      3660      3670& nbsp     3680      3690      3700 TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTG ATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAAC

 
      3710      3720      3730      3740      3750      3760      3770& nbsp     3780      3790      3800 CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGA GAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACT

 
      3810      3820      3830      3840      3850      3860      3870& nbsp     3880      3890      3900 GAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC CTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAG

 
      3910      3920      3930      3940      3950      3960      3970& nbsp     3980      3990      4000 GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG CCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCAC

 
      4010      4020      4030      4040      4050      4060      4070& nbsp     4080      4090      4100 CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCA GACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGT

 
      4110      4120      4130      4140      4150      4160      4170& nbsp     4180      4190      4200 TGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC ACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCG

 
      4210      4220      4230      4240      4250      4260      4270& nbsp     4280      4290      4300 AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCC TTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGG

 
      4310      4320      4330      4340      4350      4360      4370& nbsp     4380      4390      4400 TTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAT AAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATA

 
      4410      4420      4430      4440      4450      4460      4470& nbsp     4480      4490      4500 CGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCA GCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGT

 
      4510      4520      4530      4540      4550      4560      4570& nbsp     4580      4590      4600 GACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAA CTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTT

 
      4610      4620      4630      4640      4650      4660      4670& nbsp     4680      4690      4700 TCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTT AGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAA

 
      4710      4720      4730      4740      4750      4760      4770& nbsp     4780      4790      4800 GCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATA CGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTAT

 
      4810      4820      4830      4840      4850      4860      4870& nbsp     4880      4890      4900 CCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGG GGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACC

 
      4910      4920      4930      4940      4950      4960      4970& nbsp     4980      4990      5000 CTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCAC GACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGAACCFFAAGCACGTG

 
      5010      5020      5030      5040      5050      5060      5070& nbsp     5080      5090      5100 ACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGG TGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCC

 
      5110      5120      5130      5140      5150      5160      5170& nbsp     5180      5190      5200 TATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT ATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGA

 
      5210      5220      5230      5240      5250      5260      5270& nbsp     5280      5290      5300 GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCC CTGAACTCGCAGCTAAAAACACTACGAGCAGTAACCFFGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGG

 
      5310      5320      5330      5340      5350      5360      5370& nbsp     5380      5390      5400 TTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACC AAAACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGG

 
      5410      5420      5430 5435
GAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA
CTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCT