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84: Chehade KA, Kiegiel K, Isaacs RJ, Pickett JS, Bowers KE, Fierke CA, Andres
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by protein farnesyl transferase. J Am Chem Soc. 2002 Jul 17;124(28):8206-19.
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85: Crary SM, Kurz JC, Fierke CA. Specific phosphorothioate substitutions probe
the active site of Bacillus subtilis ribonuclease P. RNA. 2002 Jul;8(7):933-47.
PubMed PMID: 12166648; PubMed Central PMCID: PMC1370310.
86: Griffiths JS, Wymer NJ, Njolito E, Niranjanakumari S, Fierke CA, Toone EJ.
Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase.
Bioorg Med Chem. 2002 Mar;10(3):545-50. PubMed PMID: 11814840.
87: Madder RD, Kim CY, Chandra PP, Doyon JB, Baird TA Jr, Fierke CA, Christianson
DW, Voet JG, Jain A. Twisted amides inferred from QSAR analysis of hydrophobicity
and electronic effects on the affinity of fluoroaromatic inhibitors of carbonic
anhydrase. J Org Chem. 2002 Jan 25;67(2):582-4. PubMed PMID: 11798333.
88: Xiao S, Scott F, Fierke CA, Engelke DR. Eukaryotic ribonuclease P: a
plurality of ribonucleoprotein enzymes. Annu Rev Biochem. 2002;71:165-89. Epub
2001 Nov 9. Review. PubMed PMID: 12045094.
89: Fierke CA, Thompson RB. Fluorescence-based biosensing of zinc using carbonic
anhydrase. Biometals. 2001 Sep-Dec;14(3-4):205-22. Review. PubMed PMID: 11831457.
90: DiTusa CA, McCall KA, Christensen T, Mahapatro M, Fierke CA, Toone EJ.
Thermodynamics of metal ion binding. 2. Metal ion binding by carbonic anhydrase
variants. Biochemistry. 2001 May 8;40(18):5345-51. PubMed PMID: 11330997.
91: DiTusa CA, Christensen T, McCall KA, Fierke CA, Toone EJ. Thermodynamics of
metal ion binding. 1. Metal ion binding by wild-type carbonic anhydrase.
Biochemistry. 2001 May 8;40(18):5338-44. PubMed PMID: 11330996.
92: Henkels CH, Kurz JC, Fierke CA, Oas TG. Linked folding and anion binding of
the Bacillus subtilis ribonuclease P protein. Biochemistry. 2001 Mar
6;40(9):2777-89. PubMed PMID: 11258888.
93: Fang XW, Yang XJ, Littrell K, Niranjanakumari S, Thiyagarajan P, Fierke CA,
Sosnick TR, Pan T. The Bacillus subtilis RNase P holoenzyme contains two RNase P
RNA and two RNase P protein subunits. RNA. 2001 Feb;7(2):233-41. PubMed PMID:
11233980; PubMed Central PMCID: PMC1370081.
94: Hightower KE, Casey PJ, Fierke CA. Farnesylation of nonpeptidic thiol
compounds by protein farnesyltransferase. Biochemistry. 2001 Jan
30;40(4):1002-10. PubMed PMID: 11170422.
95: Jackman JE, Raetz CR, Fierke CA. Site-directed mutagenesis of the bacterial
metalloamidase UDP-(3-O-acyl)-N-acetylglucosamine deacetylase (LpxC).
Identification of the zinc binding site. Biochemistry. 2001 Jan 16;40(2):514-23.
PubMed PMID: 11148046.
96: Wymer N, Buchanan LV, Henderson D, Mehta N, Botting CH, Pocivavsek L, Fierke
CA, Toone EJ, Naismith JH. Directed evolution of a new catalytic site in
2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli. Structure. 2001
Jan 10;9(1):1-9. PubMed PMID: 11342129.
97: Cox JD, Hunt JA, Compher KM, Fierke CA, Christianson DW. Structural influence
of hydrophobic core residues on metal binding and specificity in carbonic
anhydrase II. Biochemistry. 2000 Nov 14;39(45):13687-94. PubMed PMID: 11076507.
98: Spence RA, Hightower KE, Terry KL, Beese LS, Fierke CA, Casey PJ. Conversion
of Tyr361 beta to Leu in mammalian protein farnesyltransferase impairs product
release but not substrate recognition. Biochemistry. 2000 Nov 14;39(45):13651-9.
PubMed PMID: 11076503.
99: Saderholm MJ, Hightower KE, Fierke CA. Role of metals in the reaction
catalyzed by protein farnesyltransferase. Biochemistry. 2000 Oct
10;39(40):12398-405. PubMed PMID: 11015220.
100: Kurz JC, Fierke CA. Ribonuclease P: a ribonucleoprotein enzyme. Curr Opin
Chem Biol. 2000 Oct;4(5):553-8. Review. PubMed PMID: 11006544.
101: McCall KA, Fierke CA. Colorimetric and fluorimetric assays to quantitate
micromolar concentrations of transition metals. Anal Biochem. 2000 Sep
10;284(2):307-15. PubMed PMID: 10964414.
102: Ziehler WA, Day JJ, Fierke CA, Engelke DR. Effects of 5' leader and 3'
trailer structures on pre-tRNA processing by nuclear RNase P. Biochemistry. 2000
Aug 15;39(32):9909-16. PubMed PMID: 10933810.
103: Doyon JB, Hansen EA, Kim CY, Chang JS, Christianson DW, Madder RD, Voet JG,
Baird TA Jr, Fierke CA, Jain A. Linear free energy relationships implicate three
modes of binding for fluoroaromatic inhibitors to a mutant of carbonic anhydrase
II. Org Lett. 2000 Aug 10;2(16):2557-8. PubMed PMID: 10956546.
104: Doyon JB, Hansen EA, Kim CY, Chang JS, Christianson DW, Madder RD, Voet JG,
Baird TA Jr, Fierke CA, Jain A. Linear free energy relationships implicate three
modes of binding for fluoroaromatic inhibitors to a mutant of carbonic anhydrase
II. Org Lett. 2000 May 4;2(9):1189-92. Erratum in: Org Lett 2000 Aug
10;2(16):2557. PubMed PMID: 10810704.
105: McCall KA, Huang C, Fierke CA. Function and mechanism of zinc
metalloenzymes. J Nutr. 2000 May;130(5S Suppl):1437S-46S. Review. PubMed PMID:
10801957.
106: Jackman JE, Fierke CA, Tumey LN, Pirrung M, Uchiyama T, Tahir SH, Hindsgaul
O, Raetz CR. Antibacterial agents that target lipid A biosynthesis in
gram-negative bacteria. Inhibition of diverse
UDP-3-O-(r-3-hydroxymyristoyl)-n-acetylglucosamine deacetylases by substrate
analogs containing zinc binding motifs. J Biol Chem. 2000 Apr 14;275(15):11002-9.
PubMed PMID: 10753902.
107: Burton RE, Hunt JA, Fierke CA, Oas TG. Novel disulfide engineering in human
carbonic anhydrase II using the PAIRWISE side-chain geometry database. Protein
Sci. 2000 Apr;9(4):776-85. PubMed PMID: 10794421; PubMed Central PMCID:
PMC2144608.
108: Huang C, Hightower KE, Fierke CA. Mechanistic studies of rat protein
farnesyltransferase indicate an associative transition state. Biochemistry. 2000
Mar 14;39(10):2593-602. PubMed PMID: 10704208.
109: Thompson RB, Whetsell WO Jr, Maliwal BP, Fierke CA, Frederickson CJ.
Fluorescence microscopy of stimulated Zn(II) release from organotypic cultures of
mammalian hippocampus using a carbonic anhydrase-based biosensor system. J
Neurosci Methods. 2000 Mar 1;96(1):35-45. PubMed PMID: 10704669.
110: Hunt JA, Lesburg CA, Christianson DW, Thompson RB, Fierke CA. Active-site
engineering of carbonic anhydrase and its application to biosensors. EXS.
2000;(90):221-40. Review. PubMed PMID: 11268518.
111: Alber BE, Colangelo CM, Dong J, Stålhandske CM, Baird TT, Tu C, Fierke CA,
Silverman DN, Scott RA, Ferry JG. Kinetic and spectroscopic characterization of
the gamma-carbonic anhydrase from the methanoarchaeon Methanosarcina thermophila.
Biochemistry. 1999 Oct 5;38(40):13119-28. PubMed PMID: 10529183.
112: Hunt JA, Ahmed M, Fierke CA. Metal binding specificity in carbonic anhydrase
is influenced by conserved hydrophobic core residues. Biochemistry. 1999 Jul
13;38(28):9054-62. PubMed PMID: 10413479.
113: Hightower KE, Fierke CA. Zinc-catalyzed sulfur alkyation:insights from
protein farnesyltransferase. Curr Opin Chem Biol. 1999 Apr;3(2):176-81. Review.
PubMed PMID: 10226042.
114: Jackman JE, Raetz CR, Fierke CA.
UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase of Escherichia
coli is a zinc metalloenzyme. Biochemistry. 1999 Feb 9;38(6):1902-11. PubMed
PMID: 10026271.
115: Thompson RB, Maliwal BP, Fierke CA. Selectivity and sensitivity of
fluorescence lifetime-based metal ion biosensing using a carbonic anhydrase
transducer. Anal Biochem. 1999 Feb 1;267(1):185-95. PubMed PMID: 9918670.
116: Ogura T, Inoue K, Tatsuta T, Suzaki T, Karata K, Young K, Su LH, Fierke CA,
Jackman JE, Raetz CR, Coleman J, Tomoyasu T, Matsuzawa H. Balanced biosynthesis
of major membrane components through regulated degradation of the committed
enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia
coli. Mol Microbiol. 1999 Feb;31(3):833-44. PubMed PMID: 10048027.
117: Niranjanakumari S, Stams T, Crary SM, Christianson DW, Fierke CA. Protein
component of the ribozyme ribonuclease P alters substrate recognition by directly
contacting precursor tRNA. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15212-7.
PubMed PMID: 9860948; PubMed Central PMCID: PMC28022.
118: Zimmerman KK, Scholten JD, Huang CC, Fierke CA, Hupe DJ. High-level
expression of rat farnesyl:protein transferase in Escherichia coli as a
translationally coupled heterodimer. Protein Expr Purif. 1998 Dec;14(3):395-402.
PubMed PMID: 9882574.
119: Thompson RB, Maliwal BP, Feliccia VL, Fierke CA, McCall K. Determination of
picomolar concentrations of metal ions using fluorescence anisotropy: biosensing
with a "reagentless" enzyme transducer. Anal Chem. 1998 Nov 15;70(22):4717-23.
PubMed PMID: 9844569.
120: Hightower KE, Huang CC, Casey PJ, Fierke CA. H-Ras peptide and protein
substrates bind protein farnesyltransferase as an ionized thiolate. Biochemistry.
1998 Nov 3;37(44):15555-62. PubMed PMID: 9799520.
121: Loria A, Niranjanakumari S, Fierke CA, Pan T. Recognition of a pre-tRNA
substrate by the Bacillus subtilis RNase P holoenzyme. Biochemistry. 1998 Nov
3;37(44):15466-73. PubMed PMID: 9799509.
122: Niranjanakumari S, Kurz JC, Fierke CA. Expression, purification and
characterization of the recombinant ribonuclease P protein component from
Bacillus subtilis. Nucleic Acids Res. 1998 Jul 1;26(13):3090-6. PubMed PMID:
9628904; PubMed Central PMCID: PMC147689.
123: Crary SM, Niranjanakumari S, Fierke CA. The protein component of Bacillus
subtilis ribonuclease P increases catalytic efficiency by enhancing interactions
with the 5' leader sequence of pre-tRNAAsp. Biochemistry. 1998 Jun
30;37(26):9409-16. PubMed PMID: 9649323.
124: Thompson RB, Maliwal BP, Fierke CA. Expanded dynamic range of free zinc ion
determination by fluorescence anisotropy. Anal Chem. 1998 May 1;70(9):1749-54.
doi: 10.1021/ac971199+. PubMed PMID: 21651269.
125: Stams T, Niranjanakumari S, Fierke CA, Christianson DW. Ribonuclease P
protein structure: evolutionary origins in the translational apparatus. Science.
1998 May 1;280(5364):752-5. PubMed PMID: 9563955.
126: Kurz JC, Niranjanakumari S, Fierke CA. Protein component of Bacillus
subtilis RNase P specifically enhances the affinity for precursor-tRNAAsp.
Biochemistry. 1998 Feb 24;37(8):2393-400. PubMed PMID: 9485387.
127: Lesburg CA, Huang C, Christianson DW, Fierke CA. Histidine --> carboxamide
ligand substitutions in the zinc binding site of carbonic anhydrase II alter
metal coordination geometry but retain catalytic activity. Biochemistry. 1997 Dec
16;36(50):15780-91. PubMed PMID: 9398308.
128: Hunt JA, Fierke CA. Selection of carbonic anhydrase variants displayed on
phage. Aromatic residues in zinc binding site enhance metal affinity and
equilibration kinetics. J Biol Chem. 1997 Aug 15;272(33):20364-72. PubMed PMID:
9252341.
129: Wuebbens MW, Roush ED, Decastro CM, Fierke CA. Cloning, sequencing, and
recombinant expression of the porcine inhibitor of carbonic anhydrase: a novel
member of the transferrin family. Biochemistry. 1997 Apr 8;36(14):4327-36. PubMed
PMID: 9100029.
130: Baird TT Jr, Waheed A, Okuyama T, Sly WS, Fierke CA. Catalysis and
inhibition of human carbonic anhydrase IV. Biochemistry. 1997 Mar
4;36(9):2669-78. PubMed PMID: 9054574.
131: Huang CC, Casey PJ, Fierke CA. Evidence for a catalytic role of zinc in
protein farnesyltransferase. Spectroscopy of Co2+-farnesyltransferase indicates
metal coordination of the substrate thiolate. J Biol Chem. 1997 Jan
3;272(1):20-3. PubMed PMID: 8995218.
132: Jackman JE, Merz KM Jr, Fierke CA. Disruption of the active site solvent
network in carbonic anhydrase II decreases the efficiency of proton transfer.
Biochemistry. 1996 Dec 24;35(51):16421-8. PubMed PMID: 8987973.
133: Venters RA, Farmer BT 2nd, Fierke CA, Spicer LD. Characterizing the use of
perdeuteration in NMR studies of large proteins: 13C, 15N and 1H assignments of
human carbonic anhydrase II. J Mol Biol. 1996 Dec 20;264(5):1101-16. PubMed PMID:
9000633.
134: Beebe JA, Kurz JC, Fierke CA. Magnesium ions are required by Bacillus
subtilis ribonuclease P RNA for both binding and cleaving precursor tRNAAsp.
Biochemistry. 1996 Aug 13;35(32):10493-505. PubMed PMID: 8756706.
135: Huang CC, Lesburg CA, Kiefer LL, Fierke CA, Christianson DW. Reversal of the
hydrogen bond to zinc ligand histidine-119 dramatically diminishes catalysis and
enhances metal equilibration kinetics in carbonic anhydrase II. Biochemistry.
1996 Mar 19;35(11):3439-46. PubMed PMID: 8639494.
136: Thompson RB, Ge Z, Patchan MW, Fierke CA. Performance enhancement of
fluorescence energy transfer-based biosensors by site-directed mutagenesis of the
transducer. J Biomed Opt. 1996 Jan 1;1(1):131-137. PubMed PMID: 23014654.
137: Ippolito JA, Nair SK, Alexander RS, Kiefer LL, Fierke CA, Christianson DW.
Structure of His94-->Asp carbonic anhydrase II in a new crystalline form reveals
a partially occupied zinc binding site. Protein Eng. 1995 Oct;8(10):975-80.
PubMed PMID: 8771178.
138: Whitty A, Fierke CA, Jencks WP. Role of binding energy with coenzyme A in
catalysis by 3-oxoacid coenzyme A transferase. Biochemistry. 1995 Sep
19;34(37):11678-89. PubMed PMID: 7547900.
139: Venters RA, Huang CC, Farmer BT 2nd, Trolard R, Spicer LD, Fierke CA.
High-level 2H/13C/15N labeling of proteins for NMR studies. J Biomol NMR. 1995
Jun;5(4):339-44. PubMed PMID: 7647552.
140: Ippolito JA, Baird TT Jr, McGee SA, Christianson DW, Fierke CA.
Structure-assisted redesign of a protein-zinc-binding site with femtomolar
affinity. Proc Natl Acad Sci U S A. 1995 May 23;92(11):5017-21. PubMed PMID:
7761440; PubMed Central PMCID: PMC41839.
141: Nair SK, Krebs JF, Christianson DW, Fierke CA. Structural basis of inhibitor
affinity to variants of human carbonic anhydrase II. Biochemistry. 1995 Mar
28;34(12):3981-9. PubMed PMID: 7696263.
142: Fierke CA, Hammes GG. Transient kinetic approaches to enzyme mechanisms.
Methods Enzymol. 1995;249:3-37. PubMed PMID: 7791616.
143: Puttaraju M, Beebe JA, Niranjanakumari S, Been MD, Fierke CA. Generation and
characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P
protein. Nucleic Acids Symp Ser. 1995;(33):92-4. PubMed PMID: 8643411.
144: Kiefer LL, Fierke CA. Functional characterization of human carbonic
anhydrase II variants with altered zinc binding sites. Biochemistry. 1994 Dec
27;33(51):15233-40. PubMed PMID: 7803385.
145: Beebe JA, Fierke CA. A kinetic mechanism for cleavage of precursor tRNA(Asp)
catalyzed by the RNA component of Bacillus subtilis ribonuclease P. Biochemistry.
1994 Aug 30;33(34):10294-304. PubMed PMID: 7520753.
146: Krebs JF, Ippolito JA, Christianson DW, Fierke CA. Structural and functional
importance of a conserved hydrogen bond network in human carbonic anhydrase II. J
Biol Chem. 1993 Dec 25;268(36):27458-66. PubMed PMID: 8262987.
147: Tweedy NB, Nair SK, Paterno SA, Fierke CA, Christianson DW. Structure and
energetics of a non-proline cis-peptidyl linkage in a proline-202-->alanine
carbonic anhydrase II variant. Biochemistry. 1993 Oct 19;32(41):10944-9. PubMed
PMID: 8218160.
148: Heming TA, Vanoye CG, Stabenau EK, Roush ED, Fierke CA, Bidani A. Inhibitor
sensitivity of pulmonary vascular carbonic anhydrase. J Appl Physiol. 1993
Oct;75(4):1642-9. PubMed PMID: 8282615.
149: Kiefer LL, Krebs JF, Paterno SA, Fierke CA. Engineering a cysteine ligand
into the zinc binding site of human carbonic anhydrase II. Biochemistry. 1993 Sep
28;32(38):9896-900. PubMed PMID: 8399158.
150: Krebs JF, Rana F, Dluhy RA, Fierke CA. Kinetic and spectroscopic studies of
hydrophilic amino acid substitutions in the hydrophobic pocket of human carbonic
anhydrase II. Biochemistry. 1993 May 4;32(17):4496-505. PubMed PMID: 8485128.
151: Alexander RS, Kiefer LL, Fierke CA, Christianson DW. Engineering the zinc
binding site of human carbonic anhydrase II: structure of the His-94-->Cys
apoenzyme in a new crystalline form. Biochemistry. 1993 Feb 16;32(6):1510-8.
PubMed PMID: 8431430.
152: Krebs JF, Fierke CA. Determinants of catalytic activity and stability of
carbonic anhydrase II as revealed by random mutagenesis. J Biol Chem. 1993 Jan
15;268(2):948-54. PubMed PMID: 8419374.
153: Roush ED, Fierke CA. Purification and characterization of a carbonic
anhydrase II inhibitor from porcine plasma. Biochemistry. 1992 Dec
15;31(49):12536-42. PubMed PMID: 1463741.
154: Adams JA, Fierke CA, Benkovic SJ. The function of amino acid residues
contacting the nicotinamide ring of NADPH in dihydrofolate reductase from
Escherichia coli. Biochemistry. 1991 Nov 19;30(46):11046-54. PubMed PMID:
1834173.
155: Fierke CA, Calderone TL, Krebs JF. Functional consequences of engineering
the hydrophobic pocket of carbonic anhydrase II. Biochemistry. 1991 Nov
19;30(46):11054-63. PubMed PMID: 1657158.
156: Krebs JF, Fierke CA, Alexander RS, Christianson DW. Conformational mobility
of His-64 in the Thr-200----Ser mutant of human carbonic anhydrase II.
Biochemistry. 1991 Sep 24;30(38):9153-60. PubMed PMID: 1909891.
157: Nair SK, Calderone TL, Christianson DW, Fierke CA. Altering the mouth of a
hydrophobic pocket. Structure and kinetics of human carbonic anhydrase II mutants
at residue Val-121. J Biol Chem. 1991 Sep 15;266(26):17320-5. PubMed PMID:
1910042.
158: Venters RA, Calderone TL, Spicer LD, Fierke CA. Uniform 13C isotope labeling
of proteins with sodium acetate for NMR studies: application to human carbonic
anhydrase II. Biochemistry. 1991 May 7;30(18):4491-4. PubMed PMID: 1902380.
159: Andrews J, Fierke CA, Birdsall B, Ostler G, Feeney J, Roberts GC, Benkovic
SJ. A kinetic study of wild-type and mutant dihydrofolate reductases from
Lactobacillus casei. Biochemistry. 1989 Jul 11;28(14):5743-50. PubMed PMID:
2505841.
160: Fierke CA, Benkovic SJ. Probing the functional role of threonine-113 of
Escherichia coli dihydrofolate reductase for its effect on turnover efficiency,
catalysis, and binding. Biochemistry. 1989 Jan 24;28(2):478-86. PubMed PMID:
2496745.
161: Benkovic SJ, Fierke CA, Naylor AM. Insights into enzyme function from
studies on mutants of dihydrofolate reductase. Science. 1988 Mar
4;239(4844):1105-10. Review. PubMed PMID: 3125607.
162: Fierke CA, Johnson KA, Benkovic SJ. Construction and evaluation of the
kinetic scheme associated with dihydrofolate reductase from Escherichia coli.
Biochemistry. 1987 Jun 30;26(13):4085-92. PubMed PMID: 3307916.
163: Fierke CA, Kuchta RD, Johnson KA, Benkovic SJ. Implications for enzymic
catalysis from free-energy reaction coordinate profiles. Cold Spring Harb Symp
Quant Biol. 1987;52:631-8. PubMed PMID: 3331348.
164: Mayer RJ, Chen JT, Taira K, Fierke CA, Benkovic SJ. Importance of a
hydrophobic residue in binding and catalysis by dihydrofolate reductase. Proc
Natl Acad Sci U S A. 1986 Oct;83(20):7718-20. PubMed PMID: 3463995; PubMed
Central PMCID: PMC386792.
165: Fierke CA, Jencks WP. Two functional domains of coenzyme A activate
catalysis by coenzyme A transferase. Pantetheine and adenosine 3'-phosphate
5'-diphosphate. J Biol Chem. 1986 Jun 15;261(17):7603-6. PubMed PMID: 3458707.
166: Chen JT, Mayer RJ, Fierke CA, Benkovic SJ. Site-specific mutagenesis of
dihydrofolate reductase from Escherichia coli. J Cell Biochem. 1985;29(2):73-82.
PubMed PMID: 3905833.
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